The eDNAqua-Plan project aims to advance the integration of eDNA-based aquatic biodiversity monitoring into policy by reviewing the current landscape of reference libraries, dataset repositories and metadata standards. By identifying gaps, it will provide a blueprint for an improved reference database- and eDNA landscape that is reliable (e.g. verifiable taxonomies and reporting on data collection and processing mehtods), accessible, and interoperable across markers, taxa and data types. The result will be a plan for an interlinked digital ecosystem, that can be easily adopted by new practitioners, and that will make eDNA biodiversity monitoring more transparent and reproducible across Europe.